top of page



Berlak M, Tucker E, Dorel M, Winkler A, McGearey A, Rodriguez-Fos E, Martins da Costa B, Barker K, Fyle E, Calton E, Eising S, Ober K, Hughes D, Koutroumanidou E, Carter P, Stankunaite R, Proszek P, Jain N, Rosswog C, Dorado-Garcia H, Molenaar J, Hubank M, Barone G, Anderson J, Lang P, Deubzer HE, Künkele A, Fischer M, Eggert A, Kloft C, Henssen AG, Boettcher M, Hertwig F, Blüthgen N, Chesler L, Schulte JH (2022) Mutations in ALK signaling pathways conferring resistance to ALK 1 inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells. MOLECULAR CANCER. Pubmed


Genolet O, Monaco AA, Dunkel I, Boettcher M, Schulz EG (2021) Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach. GENOME BIOLOGY. Pubmed


Bratovič M, Fonfara I, Chylinski K, Gálvez E, Sullivan T, Boerno S, Timmermann B, Boettcher M, Charpentier E (2020) Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches. NATURE CHEMICAL BIOLOGY. Pubmed

Covarrubias S, Cortez Vollmers A, Capili A, Boettcher M, Shulkin A, Ramos Correa M, Halasz H, Robinson EK, O'Brian L, Vollmers C, Blau J, Katzman S, McManus MT, Carpenter S (2020) High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways. CELL REPORTS. Pubmed

Pirona AC, Oktriani R, Boettcher M*, Hoheisel JD* (2020) Process for an efficient lentiviral cell transduction. BIOLOGY METHODS AND PROTOCOLS (*co-corresponding). Pubmed

Tromp AT, Van Gent M, Jansen JP, Scheepmaker LM, Velthuizen A, De Haas CJC, Van Kessel KPM, Bardoel BW, Boettcher M, McManus MT, Van Strijp JAG, Lebbink RJ, Haas PJA, Spaan AN (2020) Host-receptor post-translational modifications refine staphylococcal leukocidin cytotoxicity. TOXINS, 12(2), 106. Pubmed



Boettcher M*, Covarrubias S*, Biton A*, Blau J, Wang H, Zaitlen N, McManus MT (2019) Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding. BMC GENOMICS, 20: 107 (*equal contribution). Pubmed



Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT (2018) Dual gene activation and knockout screen reveals directional dependencies in genetic networks. NATURE BIOTECHNOLOGY, 36: 170-178. Pubmed


Shang W*, Jiang Y*, Boettcher M*, Ding K, Mollenauer M, Liu Z, Wen X, Liu C, Hao P, Zhao S, McManus MT, Wei L, Weiss A, Wang H (2018) Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115: E4051-E4060 (*equal contribution). Pubmed


Tromp AT, Van Gent M, Abrial P, Martin A, Jansen JP, De Haas CJC, Van Kessel KPM, Bardoel BW, Kruse E, Bourdonnay E, Boettcher M, McManus MT, Day CJ, Jennings MP, Lina G, Vandenesch F, Van Strijp JAG, Lebbink RJ, Haas PJA, Henry T, Spaan AN (2018) Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin. NATURE MICROBIOLOGY, 3: 708-717. Pubmed


Slabicki M, Lee KS, Jethwa A, Sellner L, Sacco F, Walther T, Hüllein J, Dietrich S, Wu B, Lipka DB, Oakes CC, Mamidi S, Pyrzynska B, Winiarska M, Oles M, Seifert M, Plass C, Kirschfink M, Boettcher M, Golab J, Huber W, Fröhling S, Zenz T (2016) Dissection of CD20 regulation in lymphoma using RNAi. LEUKEMIA, 30: 2409-2412. Pubmed


Boettcher M and McManus MT (2015) Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR. MOLECULAR CELL, 58: 575-85. Review. Pubmed


Boettcher M, Lawson A, Ladenburger V, Fredebohm J, Wolf J, Hoheisel JD, Frezza C, Shlomi T (2014) High throughput synthetic lethality screen reveals a tumorigenic role of adenylate cyclase in fumarate hydratase-deficient cancer cells. BMC GENOMICS, 15: 158. Pubmed


Wolf J, Müller-Decker K, Flechtenmacher C, Zhang F, Shahmoradgoli M, Mills GB, Hoheisel JD, Boettcher M (2014) An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation. ONCOGENE, 33: 4273-8. Pubmed


Fredebohm J, Wolf J, Hoheisel JD, Boettcher M (2013) Depletion of RAD17 sensitizes pancreatic cancer cells to gemcitabine. JOURNAL OF CELL SCIENCE, 126: 3380-9. Pubmed


Wolf J, Dewi DL, Fredebohm J, Müller-Decker K, Flechtenmacher C, Hoheisel JD, Boettcher M (2013) A mammosphere formation RNAi screen reveals that ATG4A promotes a breast cancer stem-like phenotype. BREAST CANCER RESEARCH, 15: R109. Pubmed


Fredebohm J, Boettcher M, Eisen C, Gaida MM, Heller A, Keleg S, Tost J, Greulich-Bode KM, Hotz-Wagenblatt A, Lathrop M, Giese NA, Hoheisel JD (2012) Establishment and characterization of a highly tumourigenic and cancer stem cell enriched pancreatic cancer cell line as a well defined model system. PLoS One, 7: e48503. Pubmed

Shenfeld M, Hachmo Y, Frenkel M, Dafni N, Boettcher M, Hoheisel JD, Dotan I, Canaani D (2012) ER-alpha-cDNA as part of a bicistronic transcript gives rise to high frequency, long term, receptor expressing cell clones. PLoS One, 7: e31977. Pubmed


Boettcher M and Hoheisel JD (2010) Pooled RNAi Screens - Technical and Biological Aspects. CURRENT GENOMICS, 11: 162-7. Review. Pubmed


Boettcher M, Fredebohm J, Gholami AM, Hachmo Y, Dotan I, Canaani D, Hoheisel JD (2010) Decoding pooled RNAi screens by means of barcode tiling arrays. BMC GENOMICS, 11: 7. Pubmed


Boettcher M, Kischkel F, Hoheisel JD (2010) High-definition DNA methylation profiles from breast and ovarian carcinoma cell lines with differing doxorubicin resistance. PLoS One, 5: e11002. Pubmed

bottom of page